초록
<P><B>Abstract</B></P> <P>Trace elements are one of the important parameters for dark fermentative H<SUB>2</SUB> production because they work as co-factors in H<SUB>2</SUB> formation biochemistry. Lack or excess of trace element and its concentrations could be an important reason for the low yield of H<SUB>2</SUB> production. In this study, the effects of 11 different trace elements (Fe, Ni, Zn, Co, Cu, Mn, Al, B, Se, Mo and W) were tested at two levels in terms of biohydrogen production from Fruit and Vegetable Wastes (FVW) with Biochemical Hydrogen Potential (BHP) Tests using Plackett-Burman statistical design. 1.1–2.8 times enhancement of biohydrogen production was determined with its addition. The most effective trace elements were found as Zn and Ni. In order to reveal the resident microbial flora, Denaturing Gradient Gel Electrophoresis (DGGE) analysis was carried out on all BHP effluent samples. Results of DGGE analysis, four microbial sequences evaluated as <I>Clostridium</I> sp.<I>, Clostridium baratii, Uncultured bacterium, Uncultured Streptococcus</I> sp., and their similarity rates were 99%, 100%, 89%, 98%, respectively.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Trace element concentrations are very important for biochemistry of biohydrogen production. </LI> <LI> Zn and Ni are the main trace elements for a successful hydrogen production from fruit and vegetable wastes. </LI> <LI> Trace element addition enhances biohydrogen production. </LI> <LI> The majority of microbial profiles obtained from this study mainly consist of <I>Clostridium</I> species. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>