BioChem - DOS

Enhancing the value of nitrogen from rapeseed meal for microbial oil production

Enzyme and microbial technology

Uckun Kiran, E.; Salakkam, A.; Trzcinski, A.P.; Bakir, U.; Webb, C.

Rapeseed meal, a major byproduct of biodiesel production, has been used as a low-cost raw material for the production of a generic microbial feedstock through a consolidated bioconversion process. Various strategies were tested for the production of a novel fermentation medium, rich in free amino nitrogen (FAN): commercial enzymes (CEs) (2.7mgg<SUP>-1</SUP> dry meal), liquid state fungal pre-treatment (LSF) using Aspergillus oryzae (4.6mgg<SUP>-1</SUP>), liquid state fungal pre-treatment followed by fungal autolysis (LSFA) (9.13mgg<SUP>-1</SUP>), liquid state pre-treatment using fungal enzymatic broth (EB) (2.1mgg<SUP>-1</SUP>), but the best strategy was a solid state fungal pre-treatment followed by fungal autolysis (34.5mgg<SUP>-1</SUP>). The bioavailability of the nitrogen sources in the novel medium was confirmed in fed-batch bioreactor studies, in which 82.3g dry cell L<SUP>-1</SUP> of the oleaginous yeast Rhodosporidium toruloides Y4 was obtained with a lipid content of 48%. The dry cell weight obtained was higher than that obtained using conventional yeast extract, due to a higher total nitrogen content in the novel biomedium. The fatty acids obtained from the microbial oil were similar to those derived from rapeseed oil.

2012

IPC Science and Technology Press ; Elsevier Science Ltd

0141-0229

1879-0909

50

6

pp.337-342

10.1016/j.enzmictec.2012.03.004

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART62506946

https://dr.ntu.edu.sg/bitstream/10356/83332/1/2012%20_%20Enhancing%20the%20value%20of%20nitrogen%20from%20rapeseed%20meal%20for%20microbial%20oil%20production.pdf

Rapeseed meal; Fermentation; Solid state fermentation; Free amino nitrogen; Rhodosporidium toruloides; Microbial oil