BioChem - DOS

Engineering alternative isobutanol production platforms

AMB Express

Felpeto-Santero, Carmen; Rojas, Antonia; Tortajada, Marta; Galá n, Beatriz; Ramó n, Daniel; Garcí a, José L

<P>A synthetic inducible operon (IbPSO) expressing <I>alsS</I>, <I>ilvC, ilvD</I> and <I>kivD</I> genes encoding a pathway capable to transform pyruvate into 2-isobutyraldehyde has been designed and two recombinant plasmids named pIZIbPSO and p424IbPSO were constructed. The IbPSO containing plasmids can generate in a single transformation event new recombinant isobutanol producer strains and are useful for testing as suitable hosts wild type bacteria in different culture media. In this way we found that <I>Shimwellia blattae</I> (p424IbPSO) was able to produce in flasks up to 6&nbsp;g&nbsp;l<SUP>&#x2212;1</SUP> of isobutanol using glucose as carbon source. Moreover, for the first time, we have demonstrated that isobutanol can be produced from sucrose using <I>Escherichia coli</I> W (ATCC9367) transformed with pIZIbPSO. These robust recombinant strains were also able to produce isobutanol from a raw carbon source like hydrolysed lignocellulosic biomass.</P>

2015

Springer Berlin Heidelberg

cc-by

2191-0855

5

1

pp.32

10.1186/s13568-015-0119-2

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART74198761

https://amb-express.springeropen.com/counter/pdf/10.1186/s13568-015-0119-2

Isobutanol; Sucrose; Synthetic operon; E. coli; S. blattae