초록
Seven zygomycetes tempe isolates were screened for ethanol production at 40<SUP>o</SUP>C, 45<SUP>o</SUP>C, 50<SUP>o</SUP>C. All the isolates were able to produce ethanol at 40<SUP>o</SUP>C with the yields of 0.39-0.47g/g on glucose and three isolates were able to produce ethanol at 45<SUP>o</SUP>C with the yields of 0.22-0.26g/g on glucose. No isolate could grow and produce ethanol at 50<SUP>o</SUP>C. One isolate was selected and used for ethanol production from untreated and fungal-pretreated oil palm empty fruit bunch (OPEFB), untreated and fungal-pretreated oat straw as well as Avicel by non-isothermal simultaneous saccharification and fermentation (NSSF). This NSSF included hydrolysis with cellulase and β-glucosidase for 24h at 45<SUP>o</SUP>C, followed by simultaneous saccharification and fermentation using the selected isolate at 40<SUP>o</SUP>C for 96h. The NSSF of untreated and fungal-pretreated OPEFB, untreated and fungal-pretreated oat straw, and Avicel resulted in 14.10%, 14.25%, 27.28%, 49.88%, and 63.77% ethanol compared to the theoretical yield.