BioChem - DOS

Continuous photofermentative production of hydrogen by immobilized Rhodobacter sphaeroides O.U.001

International journal of hydrogen energy

Zagrodnik, R.; Seifert, K.; Stodolny, M.; Laniecki, M.

Photofermentative hydrogen production applying Rhodobacter sphaeroides O.U.001 immobilized on porous glass plates or porous and non-porous glass beads in presence of malic acid (Biebl and Pfennig medium) was studied under continuous and semicontinuous conditions. The applied bacteria operating under continuous regime showed the best performance and stability while immobilized on porous glass plate modified with (3-(2-aminoethyl)aminopropyl)trimetoxysilane (AEAPTMS). Here, the total amount of produced hydrogen was 4.19 L/reactor whereas average hydrogen production rate was 12.7 mL/L medium/h at hydraulic retention time (HRT) of 22 h. For experiments in suspension cumulative hydrogen amount was only 2.39 L/reactor. Accumulation of VFA's (mainly formic acid) in reaction medium as well as accumulation of biomass on supports was responsible for the decreasing activity. Comparison of different parameters in experiments with porous and non-porous glass beads proved that HRT plays a crucial role in photofermentative hydrogen production.

2015

Pergamon Press ; Elsevier Science Ltd

0360-3199

1879-3487

40

15

pp.5062-5073

10.1016/j.ijhydene.2015.02.079

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART71904578

Biohydrogen; Rhodobacter sphaeroides; Immobilization; Photofermentation; Continuous operation