BioChem - DOS

Simultaneous biohydrogen (H2) and bioplastic (poly-β-hydroxybutyrate-PHB) productions under dark, photo, and subsequent dark and photo fermentation utilizing various wastes

International journal of hydrogen energy

Dinesh, Gujuluva Hari; Nguyen, Dinh Duc; Ravindran, B.; Chang, Soon Woong; Vo, Dai-Viet N.; Bach, Quang-Vu; Tran, Hai Nguyen; Basu, M. Jothi; Mohanrasu, Kulanthaisamy; Murugan, Ramu Satheesh; Swetha, T. Angelin; Sivapraksh, G.; Selvaraj, Arokiyaraj; Arun, A.

<P><B>Abstract</B></P> <P>The present study is focused on bio hydrogen (H<SUB>2</SUB>) and bioplastic (i.e., poly-&beta;-hydroxybutyrate; PHB) productions utilizing various wastes under dark fermentation, photo fermentation and subsequent dark-photo fermentation. Potential bio H<SUB>2</SUB> and PHB producing microbes were enriched and isolated. The effects of substrate (rice husk hydrolysate, rice straw hydrolysate, dairy industry wastewater, and rice mill wastewater) concentration (10&ndash;100%) and pH (5.5&ndash;8.0) were examined in the batch mode under the dark and photo fermentation conditions. Using 100% rice straw hydrolysate at pH 7, the maximum bio H<SUB>2</SUB> (1.53 &plusmn; 0.04 mol H<SUB>2</SUB>/mol glucose) and PHB (9.8 &plusmn; 0.14 g/L) were produced under dark fermentation condition by <I>Bacillus cereus</I>. In the subsequent dark-photo fermentation, the highest amounts of bio H<SUB>2</SUB> and PHB were recorded utilizing 100% rice straw hydrolysate (1.82 &plusmn; 0.01 mol H<SUB>2</SUB>/mol glucose and 19.15 &plusmn; 0.25 g/L PHB) at a pH of 7.0 using <I>Bacillus cereus</I> (KR809374) and <I>Rhodopseudomonas rutila</I>. The subsequent dark-photo fermentative bio H<SUB>2</SUB> and PHB productions obtained using renewable biomass (i.e., rice husk hydrolysate and rice straw hydrolysate) can be considered with respect to the sustainable management of global energy sources and environmental issues.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Bio H<SUB>2</SUB>, PHB production by DF, PF was optimized utilizing RHH, DWW, RWW and RSH. </LI> <LI> Subsequent DF-PF fermentation offers better yield (H<SUB>2</SUB> and PHB) than DF. </LI> <LI> Higher amount of bio H<SUB>2</SUB> and PHB production were recorded at 100% RSH. </LI> <LI> PHB and H<SUB>2</SUB> production by DF-PF fermentation led to reductiofn of production cost. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

2020

Elsevier

0360-3199

1879-3487

45

10

pp.5840-5853

10.1016/j.ijhydene.2019.09.036

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART98736742

Biohydrogen; Bioplastic; Poly-β-hydroxybutyrate (PHB); Rice waste; Dairy waste