초록
In this study, hydrogen production by Rhodobacter sphaeroides RV from acetate was investigated. Ammonium sulphate and sodium glutamate were used to study the effects of nitrogen sources on photosynthetic hydrogen production. The results showed the optimal concentrations for ammonium sulphate and sodium glutamate were in the range of 0.4-0.8 g/L. Orthogonal array design was applied to optimize the hydrogen-producing conditions of the concentrations of yeast, FeSO<SUB>4</SUB> and NiCl<SUB>2</SUB>. The theoretical optimal condition for hydrogen production was as follow: yeast 0.1 g/L, FeSO<SUB>4</SUB> 100 mg/L and NiCl<SUB>2</SUB> 20 mg/L.