<P><B>Abstract</B></P> <P>This work concerns the investigation of the sequential production of lactic acid (LA) and biogas from food waste (FW). LA was produced from FW using a <I>Streptococcus</I> sp. strain via simultaneous saccharification and fermentation (SSF) and separate enzymatic hydrolysis and fermentation (SHF). Via SHF a yield of 0.33g<SUB>LA</SUB>/g<SUB>FW</SUB> (productivity 3.38g<SUB>LA</SUB>/L·h) and via SSF 0.29g<SUB>LA</SUB>/g<SUB>FW</SUB> (productivity 2.08g<SUB>LA</SUB>/L·h) was obtained. Fermentation residues and FW underwent anaerobic digestion (3wt% TS). Biogas yields were 0.71, 0.74 and 0.90Nm<SUP>3</SUP>/kg<SUB>VS</SUB> for FW and residues from SSF and SHF respectively. The innovation of the approach is considering the conversion of FW into two different products through a biorefinery concept, therefore making economically feasible LA production and valorising its fermentative residues. Finally, a mass balance of three different outlines with the aim to assess the amount of LA and biogas that may be generated within different scenarios is presented.</P> <P><B>Highlights</B></P> <P> <UL> <LI> New concept of cascadic use of food waste was investigated. </LI> <LI> SHF and SSF approaches for LA have been executed. </LI> <LI> Yield and productivity were slightly higher for SHF compared to SSF. </LI> <LI> Integration of biogas production from fermentative residues. </LI> <LI> Preliminary economic and environmental assessment of the proposed two-step biorefinery process. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>