BioChem - DOS

The production of β-glucosidases by Fusarium proliferatum NBRC109045 isolated from Vietnamese forest

AMB Express

Gao, Ziqing; Van Hop, Duong; Yen, Le Thi Hoang; Ando, Katsuhiko; Hiyamuta, Shuichi; Kondo, Ryuichiro

<P><I>Fusarium proliferatum</I> NBRC109045 is a filamentous fungus isolated from Vietnamese forest due to high production of &beta;-glucosidases. Production of the enzyme was studied on varied carbon source based mediums. The highest activity was obtained in medium containing 1% corn stover + 1% wheat bran (3.31 ± 0.14 U/ml). It is interesting to note that glucose (0.69 ± 0.02 U/ml) gave higher activity and just followed by cellobiose among the di- and mono-saccharides, which is generally regarded as a universal repressor of hydrolases. We improved the zymogram method to prove that in response to various carbon sources, <I>F. proliferatum</I> could express various &beta;-glucosidases. One of the &beta;-glucosidases produced by <I>F. proliferatum</I> growing in corn stover + wheat bran based medium was partially purified and proved to have high catalytic ability.</P>

2012

Springer

cc-by

2191-0855

2

1

pp.49-49

10.1186/2191-0855-2-49

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART73722806

https://amb-express.springeropen.com/counter/pdf/10.1186/2191-0855-2-49

Fusarium proliferatum; β-glucosidases; Differential expression; The translation elongation factor 1-α