BioChem - DOS

Role of malic enzyme during fatty acid synthesis in the oleaginous fungus Mortierella alpina

Applied and environmental microbiology

Hao, Guangfei; Chen, Haiqin; Wang, Lei; Gu, Zhennan; Song, Yuanda; Zhang, Hao; Chen, Wei; Chen, Yong Q.

<P>The generation of NADPH by malic enzyme (ME) was postulated to be a rate-limiting step during fatty acid synthesis in oleaginous fungi, based primarily on the results from research focusing on ME in <I>Mucor circinelloides</I>. This hypothesis is challenged by a recent study showing that leucine metabolism, rather than ME, is critical for fatty acid synthesis in <I>M. circinelloides</I>. To clarify this, the gene encoding ME isoform E from <I>Mortierella alpina</I> was homologously expressed. ME overexpression increased the fatty acid content by 30% compared to that for a control. Our results suggest that ME may not be the sole rate-limiting enzyme, but does play a role, during fatty acid synthesis in oleaginous fungi.</P>

2014

American Society for Microbiology

0099-2240

1098-5336

80

9

pp.2672-2678

10.1128/aem.00140-14

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART69536772

https://aem.asm.org/content/aem/80/9/2672.full.pdf