Microalgae starch-based bioplastics: Screening of ten strains and plasticization of unfractionated microalgae by extrusion
메타 데이터
바이오화학분류
바이오플라스틱
기타
바이오정밀화학
기타
화장품용 기능성소재
기타
의료용 화학소재
식품첨가제
논문
Microalgae starch-based bioplastics: Screening of ten strains and plasticization of unfractionated microalgae by extrusion
학술지
Carbohydrate polymers
저자명
Mathiot, Charlie; Ponge, Pauline; Gallard, Benjamin; Sassi, Jean-Franç ois; Delrue, Florian; Le Moigne, Nicolas
초록
<P><B>Abstract</B></P> <P>Microalgae were considered in this work as a new resource for developing starch-based bioplastics. Ten green microalgae strains were screened at lab-scale for their ability to produce starch. A long run (800 h) two-stage accumulation strategy was designed with successive cultivation in sulfur-replete, then sulfur-depleted medium in autotrophic conditions. Starch content was assessed on cell lysate by enzymatic digestion of extracted starch into glucose. <I>Chlamydomonas reinhardtii</I> 11-32A strain was selected as it displayed a maximum starch-to-biomass ratio of 49%w/w, 460 h after being switched to a sulfur-deprived medium. Small-scale pilot production (30 L tubular photobioreactor) with <I>C. reinhardtii</I> 11-32A yielded sufficient biomass quantity to investigate its direct plasticization with glycerol in a twin-screw extruder. Microstructural characterization confirmed the ability for starch-enriched microalgae to be homogeneously plasticized, and hence the possibility to use microalgae as a new platform for the development of bioplastics.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Ten microalgae strains were screened for intracellular starch production. </LI> <LI> Microalgae was enriched in starch up to 49%w/w in a sulfur-deprived medium. </LI> <LI> Starch concentration of 5.07 g/L was achieved in flasks after 35 days. </LI> <LI> Direct plasticization of starch-enriched microalgal biomass was performed by twin-screw extrusion. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>