초록
<P>Identification of cost‐effective cell disruption methods to facilitate lipid extraction from microalgae represents a crucial step in identifying promising biofuel‐producing species. Various cell disruption methods including autoclaving, microwave, osmotic shock, and pasteurization were tested in the microalgae <I>Chlorococcum</I> sp. MCC30, <I>Botryococcus</I> sp. MCC31, <I>Botryococcus</I> sp. MCC32, and <I>Chlorella sorokiniana</I> MIC‐G5. Lipid content (on dry weight basis) from the four cultures on day 7 ranged from 11.15 to 48.33%, and on day 14 from 11.42 to 44.26%. Among the methods tested, enhanced lipid extraction was achieved through osmotic shock (15% NaCl) for <I>Botryococcus</I> sp. MCC32, microwave (6？min) for <I>Botryococcus</I> sp. MCC31, osmotic shock (5% NaCl) for <I>Chlorella sorokiniana</I> MIC‐G5 and microwave (2？min) for <I>Chlorococcum</I> sp. MCC30. The highest palmitate (16:0) contents (25.64% and 34.20%) were recorded with osmotic shock (15% NaCl) treatment for <I>Botryococcus</I> sp. MCC32 and microwave (6？min) for <I>Botryococcus</I> sp. MCC31, respectively. Two strains, along with their respective cell disruption methods, were identified as promising oil blends or nutraceuticals due to their high unsaturated fatty acid (UFA) content: <I>Botryococcus</I> sp. MCC31 (37.6% oleic acid content; 39.37% UFA) after autoclaving and <I>Botryococcus</I> sp. MCC32 after osmotic shock of 15% NaCl treatment (19.95% oleic acid content; 38.17% UFA).</P>