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Simultaneous saccharification and fermentation of steam exploded duckweed: Improvement of the ethanol yield by increasing yeast titre

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논문

Simultaneous saccharification and fermentation of steam exploded duckweed: Improvement of the ethanol yield by increasing yeast titre

학술지

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

저자명

Zhao, X.; Moates, G.K.; Elliston, A.; Wilson, D.R.; Coleman, M.J.; Waldron, K.W.

초록

<▼1><P><B>Highlights</B></P><P>&#x2022;<P>Steam explosion of duckweed enhances SSF at low (2%&nbsp;w/v) substrate concentrations.</P>&#x2022;<P>High substrate concentrations (20%&nbsp;w/v) result in much lower ethanol yields.</P>&#x2022;<P>Ethanol yields can be considerably increased with higher yeast inoculum.</P>&#x2022;<P>Or by preconditioning yeasts in steam explosion liquor containing inhibitors.</P>&#x2022;<P>The extra/preconditioned yeast metabolise fermentation inhibitors.</P></P></▼1><▼2><P>This study investigated the conversion of <I>Lemna minor</I> biomass to bioethanol. The biomass was pre-treated by steam explosion (SE, 210&nbsp;°C, 10&nbsp;min) and then subjected to simultaneous saccharification and fermentation (SSF) using Cellic&reg; CTec 2 (20&nbsp;U or 0.87&nbsp;FPU&nbsp;g<SUP>&#x2212;1</SUP> substrate) cellulase plus &beta;-glucosidase (2&nbsp;U&nbsp;g<SUP>&#x2212;1</SUP> substrate) and a yeast inoculum of 10% (v/v or 8.0&nbsp;×&nbsp;10<SUP>7</SUP>&nbsp;cells&nbsp;mL<SUP>&#x2212;1</SUP>). At a substrate concentration of 1% (w/v) an ethanol yield of 80% (w/w, theoretical) was achieved. However at a substrate concentration of 20% (w/v), the ethanol yield was lowered to 18.8% (w/w, theoretical). Yields were considerably improved by increasing the yeast titre in the inoculum or preconditioning the yeast on steam exploded liquor. These approaches enhanced the ethanol yield up to 70% (w/w, theoretical) at a substrate concentration of 20% (w/v) by metabolising fermentation inhibitors.</P></▼2>

발행연도

2015

발행기관

Elsevier Applied Science ;, Elsevier Science Pub. Co

라이선스

cc-by-nc-nd

ISSN

0960-8524

194

페이지

pp.263-269

주제어

BG, Novozyme® 188; CE, Celluclast® 1.5; CTec 2, Cellic® CTec 2; CWMs, cell wall materials; FDM, freeze dry and freeze mill; SE, steam explosion; SSF, simultaneous saccharification and fermentation; YM, yeast and mould broth; Duckweed; Bioethanol production; Steam explosion; Cellulase; Simultaneous saccharification and fermentation

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논문; 2015-10-01

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