BioChem - DOS

Construction of homologous and heterologous synthetic sucrose utilizing modules and their application for carotenoid production in recombinant Escherichia coli

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Kim, J.R.; Kim, S.H.; Lee, S.Y.; Lee, P.C.

Sucrose is one of the most promising carbon sources for industrial fermentation. We expressed synthetic modules expressing genes of the PEP-PTS and non-PTS pathways in Escherichia coli K12 for comparison. We selected PEP-PTS pathway genes of Lactobacillus plantarum and Staphylococcus xylosus and non-PTS pathway genes of sucrose-utilizing (Scr<SUP>+</SUP>) E. coli EC3132. Switchable Scr<SUP>+</SUP> modules expressing E. coli EC3132 non-PTS genes conferred better sucrose-utilizing ability on Scr<SUP>-</SUP>E. coli K12 than E. coli EC3132. Scr<SUP>+</SUP> modules expressing S. xylosus PEP-PTS genes conferred a sucrose-utilizing ability on E. coli K12. Among L. plantarum PEP-PTS genes, SacA<SUB>LP</SUB> and SacK<SUB>LP</SUB> were functional in E. coli K12. CscA<SUB>EC</SUB>-CscB<SUB>EC</SUB>-CscK<SUB>EC</SUB> (non-PEP-PTS module) or ScrA<SUB>SX</SUB>-SacA<SUB>LP</SUB>-SacK<SUB>LP</SUB> (PEP-PTS module) was introduced to a diapolycopene-producing E. coli strain. In both Scr<SUP>+</SUP>E. coli K12, the sucrose-utilizing ability of the modules was not affected by diapolycopene formation, indicating that the modular Scr<SUP>+</SUP> systems could be employed for developing sustainable bioprocesses using sucrose.

2013

Elsevier Applied Science

0960-8524

130

pp.288-295

10.1016/j.biortech.2012.11.148

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART65520645

Sucrose; Carotenoids; Synthetic module; Metabolic engineering