Pre-treatment and enzymatic hydrolysis of lettuce residues as feedstock for bio-butanol production
메타 데이터
바이오화학분류
바이오플라스틱
플라스틱
바이오정밀화학
용매
화학제품
연료
화장품용 기능성소재
계면활성제⁄증점제
의료용 화학소재
치료제
식품첨가제
논문
Pre-treatment and enzymatic hydrolysis of lettuce residues as feedstock for bio-butanol production
학술지
Biomass & bioenergy
저자명
Procentese, Alessandra; Raganati, Francesca; Olivieri, Giuseppe; Elena Russo, Maria; Marzocchella, Antonio
초록
<P><B>Abstract</B></P> <P>The residue of the fresh-cut vegetable packaging process is a major environmental concern for food industries. Actually 50% of the processed vegetables must be disposed of. But the lettuce fraction sorted and deemed unsuitable to be bagged may be used as feedstock for butanol production because it is rich in sugars. In this work acetone butanol ethanol (ABE) was produced from enzymatic-hydrolysed <I>Lactuca sativa</I> leaves. The lettuce leaves were first pre-treated with NaOH and then hydrolysed. The NaOH concentration was increased up to 200 kg m<SUP>−3</SUP>, Cellic CTec 2 (Novozymes) was used for the hydrolysis. The sugars contained in the biomass hydrolysate were fermented in batch cultures of <I>Clostridium acetobutylicum</I> DSMZ 792. The fermentation process was characterized in terms of sugar conversion and ABE production. The hydrolysate of the NaOH-pre-treated lettuce contained glucose and xylose (about 50/50). Under optimal conditions the sugar concentration after the enzymatic hydrolysis was 19.5 g L<SUP>−1</SUP>, the ABE concentration was 1.44 g L<SUP>−1</SUP> and the butanol concentration was 1.1 g L<SUP>−1</SUP>. Some tests were also carried out with synthetic mixtures of glucose and xylose to investigate the effects of the alkaline pre-treatment on the fermentation process.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Vegetable residues from ‘fresh cut’ market were studied as feedstock for ABE fermentation. </LI> <LI> ABE was produced from enzymatic-hydrolysed of NaOH-pretreated lettuce residues. </LI> <LI> ABE fermentation with synthetic medium and hydrolysed lettuce were compared. </LI> <LI> 1.1 g L<SUP>−1</SUP> butanol was obtained from feedstock pretreated with 80 kg m<SUP>−3</SUP> NaOH. </LI> </UL> </P>