초록
The red yeast Sporobolomyces ruberrimus H110 was able to use glycerol as a carbon source. The highest concentration (0.51gL<SUP>-1</SUP>) and productivity (0.0064gL<SUP>-1</SUP>h<SUP>-1</SUP>) of carotenoids were achieved when raw glycerol from biodiesel production, containing around 1gL<SUP>-1</SUP> of fatty acids, was used as the carbon source, which represented increases of 27% and 1.5x, respectively, in relation to pure glycerol. Mass spectrometry analysis led to the identification of four carotenoids in the fermented samples, torularhodin, torulene, β-carotene and γ-carotene. The use of raw glycerol also enhanced the proportion of torularhodin (69% against 59% in pure glycerol). The addition of individual fatty acids (palmitic, stearic, oleic and linoleic acids) to pure glycerol resulted in increases between 15% and 25% in maximum concentration and between 1.6x and 2.0x in productivity of carotenoids. The presence of palmitic and oleic acids increased the torularhodin proportion to 66%.