BioChem - DOS

Enzymatic Synthesis of Lipid II and Analogues

Angewandte Chemie

Huang, Lin‐ Ya; Huang, Shih‐ Hsien; Chang, Ya‐ Chih; Cheng, Wei‐ Chieh; Cheng, Ting‐ Jen R.; Wong, Chi‐ Huey

<P><B>Abstract</B></P><P>The emergence of antibiotic resistance has prompted active research in the development of antibiotics with new modes of action. Among all essential bacterial proteins, transglycosylase polymerizes lipid&#8197;II into peptidoglycan and is one of the most favorable targets because of its vital role in peptidoglycan synthesis. Described in this study is a practical enzymatic method for the synthesis of lipid&#8197;II, coupled with cofactor regeneration, to give the product in a 50&ndash;70&thinsp;% yield. This development depends on two key steps: the overexpression of MraY for the synthesis of lipid&#8197;I and the use of undecaprenol kinase for the preparation of polyprenol phosphates. This method was further applied to the synthesis of lipid&#8197;II analogues. It was found that MraY and undecaprenol kinase can accept a wide range of lipids containing various lengths and configurations. The activity of lipid&#8197;II analogues for bacterial transglycolase was also evaluated.</P>

2014

WILEY&#x2010;VCH Verlag

0044-8249

1521-3757

126

31

pp.8198-8203

10.1002/anie.201402313

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART70014059

Enzyme; Glycolipide; Lipide; Proteine; Synthesemethoden