초록
Biofuels are of increasing interest as alternatives to the petroleum-based fuels. In particular, second and third generation advanced biofuels, that could be produced from renewable materials such as agricultural residues appear to be more competitive. In this context, this work has focused on the potential of the potato peel use (PPW), a zero value waste, as feedstock for bioethanol production. First, an economical medium for amylase production from Bacillus sp. UEB-S (amylase UEB-S) was formulated using potato peel (10gL<SUP>-1</SUP>) as a carbon source and soy flour (20gL<SUP>-1</SUP>) as a nitrogen source. Amylase production reached 15U/mL at flask scale and increased to 87U/mL at fermenter scale. A comparative study between acidic and enzymatic hydrolysis of PPW was investigated. It showed that a combined enzymatic treatment using amylase UEB-S and Amyloglucosidase under optimal conditions gave significant yields of reducing sugars (69gL<SUP>-1</SUP>) and ethanol (21gL<SUP>-1</SUP>). These results are highly interesting and demonstrate the possibility of substituting commercial enzyme by an onsite produced enzyme as well as the potential of PPW as bioethanol feedstock.