초록
A strain AS-S01a, capable of producing high-titer alkaline α-amylase, was isolated from a soil sample of Assam, India and was taxonomically identified as Bacillus subtilis strain AS-S01a. Optimized α-amylase yield by response surface method (RSM) was obtained as 799.0U with a specific activity of 201.0U/mg in a process control bioreactor. A 21.0kDa alkaline α-amylase purified from this strain showed optimum activity at 55<SUP>o</SUP>C and pH 9.0, and it produced high molecular weight oligosaccharides including small amount of glucose from starch as the end product. The K<SUB>m</SUB> and V<SUB>max</SUB> values for this enzyme towards starch were determined as 1.9mg/ml and 198.21μmol/min/mg, respectively. The purified α-amylase retained its activity in presence of oxidant, surfactants, EDTA and various commercial laundry detergents, thus advocating its suitability for various industrial applications.