Response surface methodology for the optimization of α-amylase production by Bacillus subtilis ZJF-1A5
메타 데이터
바이오화학분류
바이오정밀화학
기타
논문
Response surface methodology for the optimization of α-amylase production by Bacillus subtilis ZJF-1A5
학술지
Advanced materials research : AMR
저자명
Sun, Jun Liang; Liang, Xin Hong; Zeng, Jie; Li, Guang Lei; Sun, Jun Liang
초록
<P>The aim of this work was to optimize the process parameters through the statistical approach for the production of alpha amylase by <I>Bacillus subtilis</I> ZJF-1A5 in submerged fermentation (SMF). The process parameters influencing the enzyme production were identified by using response surface methodology. Among the variables screened, the temperature and time were most significant and showed a positive interaction. The optimum levels were: temperature 35.8 °C, pH 5.03 and time 54h and under these conditions α-amylase yield was up to 191.15 U/mL.</P>