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Partial characterization of cold active amylases and proteases of Streptomyces sp. from Antarctica

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논문

Partial characterization of cold active amylases and proteases of Streptomyces sp. from Antarctica

학술지

Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]

저자명

Cotâ rleŢ , Mihaela; NegoiŢ ă , Teodor Gh.; Bahrim, Gabriela E.; Stougaard, Peter

초록

<P>The aim of this study was to isolate novel enzyme-producing bacteria from vegetation samples from East Antarctica and also to characterize them genetically and biochemically in order to establish their phylogeny. The ability to grow at low temperature and to produce amylases and proteases cold-active was also tested. The results of the 16S rRNA gene sequence analysis showed that the 4 Alga rRNA was 100% identical to the sequences of <I>Streptomyces</I> sp. rRNA from Norway and from the Solomon Islands. The <I>Streptomyces</I> grew well in submerged system at 20°C, cells multiplication up to stationary phase being drastically increased after 120 h of submerged cultivation. The beta-amylase production reached a maximum peak after seven days, while alpha-amylase and proteases were performing biosynthesis after nine days of submerged cultivation at 20°C. Newly <I>Streptomyces</I> were able to produce amylase and proteases in a cold environment. The ability to adapt to low temperature of these enzymes could make them valuable ingredients for detergents, the food industry and bioremediation processes which require low temperatures.</P>

발행연도

2011

발행기관

Sociedade Brasileira de Microbiologia

라이선스

cc-by-nc

ISSN

1517-8382

ISSN

1678-4405

42

3

페이지

pp.868-877

주제어

Streptomyces sp.; East Antarctica; cold-adapted strain; cold active proteases and amylase

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논문; 2011-09-01

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