BioChem - DOS

Production of cellulases and xylanase by Aspergillus fumigatus SK1 using untreated oil palm trunk through solid state fermentation

Process biochemistry

Ang, S.K.; E.M., S.; Y., A.; A.A, S.; M.S, M.

Direct utilization of untreated oil palm trunk (OPT) for cellulases and xylanase production by Aspergillus fumigatus SK1 was conducted under solid-state fermentation (SSF). The highest activities of extracellular cellulases and xylanases were produced at 80% moisture level, initial pH 5.0, 1x10<SUP>8</SUP> spore/g (inoculum) with 125&mu;m of OPT as sole carbon source. The cellulases and xylanase activities obtained were 54.27, 3.36, 4.54 and 418.70U/g substrates for endoglucanase (CMCase), exoglucanase (FPase), &beta;-glucosidase and xylanase respectively. The crude cellulases and xylanase required acidic condition to retain their optimum activities (pH 4.0). Crude cellulases and xylanase were more stable at 40<SUP>o</SUP>C compared to their optimum activities conditions (60<SUP>o</SUP>C for FPase and 70<SUP>o</SUP>C for CMCase, &beta;-glucosidase and xylanase). SDS-PAGE and zymogram analysis showed that Aspergillus fumigatus SK1 could secrete cellulases (endoglucanase, exoglucanase and &beta;-glucosidase), xylanase and protease. Enzymatic degradation of alkaline treated OPT with concentrated crude cellulases and xylanases resulted in producing polyoses.

2013

Elsevier Applied Science

1359-5113

48

9

pp.1293-1302

10.1016/j.procbio.2013.06.019

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART66566749

http://psasir.upm.edu.my/id/eprint/28041/1/28041.pdf

Oil palm trunk (OPT); Cellulases; Xylanase; Solid-state fermentation; Saccharification