BioChem - DOS

Uniform culture in solid-state fermentation with fungi and its efficient enzyme production

Journal of bioscience and bioengineering

Ito, Kazunari; Kawase, Tomoka; Sammoto, Hiroyuki; Gomi, Katsuya; Kariyama, Masahiro; Miyake, Tsuyoshi

<P><B>Abstract</B></P><P>Solid-state fermentation (SSF) has attracted a lot of interest for carrying out high-level protein production in filamentous fungi. However, it has problems such as the fermentation heat generated during the culture in addition to the reduced mobility of substances. These conditions lead to a nonuniform state in the culture substrate and result in low reproducibility. We constructed a non-airflow box (NAB) with a moisture permeable fluoropolymer membrane, thereby making it possible to control and maintain uniform and optimal conditions in the substrate. For the NAB culture in <I>Aspergillus oryzae</I>, temperature and water content on/in the whole substrate were more consistent than for a traditional tray box (TB) culture. Total weight after the culture remained constant and dry conditions could be achieved during the culture. These data demonstrate the possibility of growing a uniform culture of the whole substrate for SSF. The NAB is advantageous because it allows for the control of exact temperature and water content in the substrate during the culture by allowing vapor with latent heat to dissipate out of the box. In addition, several enzymes in the NAB culture exhibited higher production levels than in the TB culture. We believe that culturing in the constructed NAB could become a standard technique for commercial SSF.</P>

2011

Elsevier

1389-1723

1347-4421

111

3

pp.300-305

10.1016/j.jbiosc.2010.11.008

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART55531027

Aspergillus oryzae; Solid-state fermentation (SSF); Non-airflow box (NAB) culture; Uniform culture; Enzyme production