BioChem - DOS

Application of different feeding strategies in fed batch culture for pullulanase production using sago starch

Carbohydrate polymers

R., S.; M.S., M.; E.M., S.; K.O., N.A.; A.A., S.; K., K.

The production of pullulanase by Bacillus flavothermus KWF-1 in batch and fed batch culture were compared using 2L bioreactor. In batch culture, 0.0803U/mL of pullulanase activity with specific activity of 0.0213U/mg was produced by controlling the agitation speed and temperature at 200rpm and 50<SUP>o</SUP>C, respectively. Fed batch production was studied by feeding the culture with different sago starch concentrations in various feeding modes for enhanced pullulanase production. Exponential feeding mode at dilution rate of 0.01/h was the preeminent strategy for enhanced pullulanase production of 0.1710U/mL with specific activity of 0.066U/mg. It had shown an increment of pullulanase production and specific activity by 2.1 and 3.1-fold, respectively when compared to batch culture. Increment of pullulanase activity in exponential feeding mode improved hydrolyzation of sago starch into maltotriose and panose by 4.5 and 2.5-fold respectively compared to batch system.

2014

Applied Science Publishers ; Elsevier Science Ltd

0144-8617

102

pp.962-969

10.1016/j.carbpol.2013.10.031

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART68597469

http://psasir.upm.edu.my/id/eprint/36508/1/Application%20of%20different%20feeding%20strategies%20in%20fed%20batch%20culture%20for%20pullulanase%20production%20using%20sago%20starch.pdf

Pullulanase; Sago; Fed batch; Maltose; Maltotriose; Panose