BioChem - DOS

Bioproduction of hydrogen by simultaneous saccharification and fermentation of cassava starch with 2-deoxyglucose-resistant mutant strains of Clostridium tyrobutyricum

International journal of hydrogen energy

Jiang, L.; Zhu, L.; Xu, X.; Lin, M.; Li, Y.; Li, X.; Cui, H.; Huang, H.

Laboratory mutagenesis of microorganisms offers the possibility of relating acquired mutations to improve the quality of microbial cultures. In the present study, a mutant strain, Clostridium tyrobutyricum ATCC 25755 DG-8, with significantly elevated α-amylase activity as well as resistant to the non-metabolizable and toxic glucose analog 2-deoxyglucose (2-DG) was obtained by implanting the low-energy nitrogen ion beam. DG-8 was further developed to produce hydrogen by simultaneous saccharification and fermentation (SSF) directly form cassava starch in batch fermentation mode, which to our knowledge is at the first attempt in genus Clostridium. Our results demonstrated that the increased activity of α-amylase would be attributed to the hydrogen over-producing. Higher hydrogen yield (3.2 mol/mol glucose) was achieved with the volumetric productivity of 0.41 L/h/L when the initial total sugar concentration of cassava starch rise up to 100 g/L. The present work will help to decrease the cost of hydrogen fermentation process and stimulate its industrial application in the near future.

2013

Pergamon Press ; Elsevier Science Ltd

0360-3199

1879-3487

38

15

pp.6349-6356

10.1016/j.ijhydene.2013.02.109

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART65928584

Hydrogen; Cassava starch; Simultaneous saccharification and fermentation; 2-Deoxyglucose-resistant mutant; Clostridium tyrobutyricum