BioChem - DOS

β-Amylase production from packaging industry wastewater using a novel strain Paenibacillus chitinolyticus CKS 1

RSC advances

Mihajlovski, Katarina R.; Radovanović , Neda R.; Miljković , Miona G.; Š iler-Marinković , Slavica; Rajilić -Stojanović , Mirjana D.; Dimitrijević -Branković , Suzana I.

<P>Transport packaging factories generate a large amount of wastewater that contains residuals of starch glue. These residuals could be used as substrates for microorganism growth and enzyme production. In this study, &beta;-amylase production using a new strain<I>Paenibacillus chitinolyticus</I>CKS1 was optimized using wastewater from a Serbian transport packaging factory. The optimization of &beta;-amylase production was carried out using Response Surface Methodology (RSM). A Central Composite Design under the RSM with four interacting parameters (incubation time, inoculum concentration, casein hydrolysate concentration and yeast extract concentration) was employed to identify the optimal conditions for maximum &beta;-amylase activity (334.20 U L<SUP>&#x2212;1</SUP>) as 62 h of incubation with 2.40% inoculum, 2.02 g L<SUP>&#x2212;1</SUP>casein hydrolysate and 3.98 g L<SUP>&#x2212;1</SUP>yeast extract. High performance liquid chromatography showed that the<I>P. chitinolyticus</I>CKS1 strain hydrolyzed starch to form maltose as a major product. Due to the application of wastewater as an inexpensive material for enzyme and maltose production it may be considered that the economic and eco-friendly aspects of this method are very promising.</P>

2015

The Royal Society of Chemistry

2046-2069

5

110

pp.90895-90903

10.1039/C5RA11964B

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